Miseq System

Methods Guide

All The Information you need, from BeadChips to library preparation to sequencer selection and analysis

AmpliSeq for Illumina Sequencing Solution

Delivery a range of ready-to-use and custom panels for simple, flexible targeted resequencing that provides high-quality data you can trust

Enabling Human Microbiome Studies

See how 16S rRNA sequencing on the MiSeq System enables studies like The American Gut Project.

The MiSeq System facilitates your research with a wide range of sequencing applications. It is capable of automated paired-end reads and up to 15 Gb per run, delivering over 600 bases of sequence data per read. The library prep kits that it uses are optimized for a variety of applications, including targeted gene, small genome, and amplicon sequencing, 16S metagenomics, and more.

Library prep kits prepare input RNA or DNA for a diverse range of applications such as whole-genome and targeted region resequencing, transcriptome analysis, and expression profiling. Each library prep method is optimized to fit each unique application, and most are available in a variety of throughput and multiplexing configurations.

The MiSeq System can go from DNA to data in just over 8 hours. A compact, all-in-one platform incorporates cluster generation, paired-end fluidics, sequencing by synthesis chemistry, and data analysis. An intuitive touch screen and plug-and-play reagents with RFID tracking add convenience. The MiSeq System eliminates the need for auxiliary hardware and computing resources, saving valuable lab bench space. Seamless data upload to BaseSpace Sequence Hub enables analysis, collaboration, and security.

System software guides you through sample sheet creation and setup prior to a run, then walks you through flow cell and reagent loading steps. Control software overviews quality statistics you can monitor as the run progresses. Once primary analysis completes, reporting software launches and automatically analyzes the data quality and coverage information of each sample.

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