Forensic Instrument

MiSeq FGx Sequencing System is the first and only next-generation sequencing (NGS) bench-top instrument developed and validated for forensic genomics. MiSeq FGx is ideal for studying genealogy, mitochondrial DNA analysis, STR and SNP with a wide variety of sample types, from high quality genomic DNA (gDNA) to degraded, mixed and limited samples..

  • Detailed DNA profiles from a wide range of sample types, from high-quality genomic DNA (gDNA) to degraded, mixed, and limited samples.
  • Prepare and sequence libraries and analyze data in a single workflow
  • Dedicated reagent kits and matched analysis software empower answers for all human identification investigations.
  • The first and only instrument to interrogate up to 96 combined SNP and STR libraries in a single run

Forensic Kits

All products below from Verogen are validated to work on the MiSeq FGx Sequencing System and analyzed in Universal Analysis Software (UAS).

ForenSeq DNA Signature Prep Kit is the only NGS-based short-tandem repeat (STR) sequencing chemistry approved for upload to the National DNA Index System (NDIS) for casework.

  • No need for multiple kit as it’s combining > 200 markers (autosomal, X- and Y-STRs, identity-informative, phenotype-informative and biogeographical ancestry-informative SNPs) into a single streamlined workflow
  • Recover more highly discriminating genetic information from heavily degraded DNA (amplicon sizes less than 200 bp)
  • Leverage the power and sensitivity to detect minor contributors at < 5% in mixed samples
  • Accept samples from gDNA, buccal swabs, FTA card

ForenSeq mtDNA Whole Genome Kit offers sequencing the whole mitochondrial genome (mtGenome) with minimal DNA input and exceptional data recovery.

  • Provide additional resolution for severely degraded samples.
  • Has the lowest list price of any commercial whole genome assay with multiplexing capability
  • Accept sample from gDNA and mtDNA extracted from hair, bone, teeth, and buccal swabs with target of 16,569 bp mtGenome
  • Deploys small amplicons sourced from the latest mitochondrial (mtDNA) databases for enhanced variant detection
  • Includes two normalization methods: high-quality genomic DNA (gDNA) through low-level and complex samples.

ForenSeq mtDNA Control Region Kit offers to interrogating the control region of the mitochondrial genome (mtGenome) with minimal DNA input and optimum sensitivity. Produces a concise data set from the entire control region for direct upload to CODIS with multiplexing capability.

  • Accept sample from gDNA and mtDNA extracted from hair, bone, teeth, and buccal swabs with target of 1200 bp mtDNA control region
  • Deploys small amplicons sourced from the latest mitochondrial (mtDNA) databases for enhanced variant detection
  • Includes two normalization methods: high-quality genomic DNA (gDNA) through low-level and complex samples.

ForenSeq Kintelligence Kit is the only sequence-based assay designed for forensic genetic genealogy (FGG) with target of 10,230 SNP markers explicitly curated for forensic kinship use. ForenSeq Kintelligence Kit minimizes privacy concerns by excluding medically informative and minor allele frequency SNPs found in whole-genome sequencing (WGS) or array-based workflows.

  • Robust PCR and primer designs allow for a single SNP multiplex that works across global populations.
  • Prepare up to 12 forensic samples per ForenSeq Kintelligence Kit, with high-quality outcomes with low-quality samples.
  • Accept sample from gDNA from hair, bone, teeth, blood, semen, and buccal swabs