HiSeq 4000 System

Choose the Right Sequencer

For tips about how to choose the best sequencer and transform your lab, download the buyer's Guide to the Next-Generation Sequencing System.

Core Facility Case Study

Learn how the UCSF Center for Advanced Technology transitioned from HiSeq 2500 System to the HiSeq 4000 System.

High Throughput Flipbook

Investigate a number of High-Throughput applications that can be run on the HiSeq Series.

The HiSeq 3000/HiSeq 4000 Sequencing Systems build on the proven performance of the HiSeq 2500 System and leverage the patterned flow cell technology of the ultra-high-throughput HiSeq X Systems to deliver high sequencing speed and performance. Offering high coverage, fast turnaround times, and the flexibility to process various sample types, the HiSeq 3000/HiSeq 4000 Systems provide a multi-application solution for production-scale genomic laboratories.

The HiSeq 3000/HiSeq 4000 Systems deliver high-throughput at a low price per sample across a range of genomic applications. The HiSeq 4000 System can sequence up to 12 genomes, 100 transcriptomes, or 96 exomes in fewer than 3.5 days. This high capacity means more samples can be sequenced simultaneously at a greater depth, generating rich and meaningful data.

The dual-flow cell HiSeq 4000 System can process one or two flow cells in parallel and provides high output and daily throughput with a capacity of 1.5 Tb and 5 billion reads per run. The HiSeq 3000 System offers the same output per flow cell as the HiSeq 4000 System, but only processes one flow cell at a time, delivering 750 Gb and 2.5 billion reads per run. When throughput needs increase, the HiSeq 3000 System can be upgraded to a HiSeq 4000 System.

Illumina offers a full range of HiSeq 3000/HiSeq 4000 software solutions to help you go from biological samples to meaningful results. BaseSpace Sequence Hub provides an integrated solution for the analysis and storage of Illumina sequencing data. With whole-genome sequencing,exome/enrichment, and RNA-Seq apps, BaseSpace Sequence Hub streamlines NGS data analysis while providing an efficient means for data storage, sharing, and transfer.

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